Protein thermodynamics: Are native proteins metastable?
نویسندگان
چکیده
منابع مشابه
Rapid photochemical cross-linking--a new tool for studies of metastable, amyloidogenic protein assemblies.
Amyloidoses comprise a class of diseases characterized pathologically by the presence of deposits of fibrillar, aberrantly folded proteins, known as amyloids. Historically, these deposits were considered the key factors causing disease. However, recent evidence suggests that soluble protein oligomers, which are precursors for amyloid fibrils, are the primary toxic effectors responsible for the ...
متن کاملMetastability of native proteins and the phenomenon of amyloid formation.
An experimental determination of the thermodynamic stabilities of a series of amyloid fibrils reveals that this structural form is likely to be the most stable one that protein molecules can adopt even under physiological conditions. This result challenges the conventional assumption that functional forms of proteins correspond to the global minima in their free energy surfaces and suggests tha...
متن کاملMETA: A computational tool for predicting metastable states in the folding pathways of proteins
The thermodynamic stabilities (∆G, free energy change) of proteins are being measured by traditional denaturation methods (∆GU, free energy of unfolding) and native hydrogen-deuterium (H/D) exchange method (∆GHX, free energy of exchange), in general. Estimation of an accurate ∆G for proteins at ambient conditions is indispensable to unambiguously address the folding events of the proteins, whic...
متن کاملCooperative Unfolding Units and Metastable States of Cytochrome c551 from Pseudomonas aeruginosa under Native Conditions
Unfolding pathways of Pseudomonas aeruginosa cytochrome c551 (Pa cyt c) characterized by native state hydrogen-deuterium exchange (NS H/D) methods in conjunction with multi-dimensional NMR techniques have been reported in the literature. Based on the analyses carried out on the H/D exchange data, it has been shown that there are four submolecular unfolding units or three cryptic intermediates (...
متن کاملProtein Stability, Folding, Disaggregation and Etiology of Conformational Malfunctions
Estimation of protein stability is important for many reasons: first providing an understanding of the basic thermodynamics of the process of folding, protein engineering, and protein stability plays important role in biotechnology especially in food and protein drug design. Today, proteins are used in many branches, including industrial processes, pharmaceutical industry, and medical fields. A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Nature chemistry
دوره 3 12 شماره
صفحات -
تاریخ انتشار 2011